Multichannel-systems Cardio2D Manual Manual de usuario

Cardio2D Manual

Cardio2D Manual 96 Single channel view and the color map of the "Heartbeat" tab correspond with each other: The excitation pattern of that

Cardio 2D+ Analyzer Software 97 Color Map Click the "Copy to Clipboard" icon to copy the color map to the clipboard. Click the "Save

Cardio2D Manual 98 When changing the propagation path from the minimum maximum default to manual, both displays in the "Latency / Velocity"

Cardio 2D+ Analyzer Software 99 Color Map: The usual used color map for cardiac issues is the "Jet Invers" map, but you can also choose &quo

Cardio2D Manual 100 Click the "Map Settings" icon . The dialog "Propagation Map Settings" appears. Click "Data" tab.

Cardio 2D+ Analyzer Software 101 Click "2D" tab in Analyzer Settings. The setting for the "Propagation Path" is from minimum to m

Cardio2D Manual 102 Select "Velocity" from the drop down menu. The diagram shows the velocity (cm/s) of the excitation in dependency of the

Cardio 2D+ Analyzer Software 103 6.7 HB Averager Click the "HB Averager" tab. The "HB Averager" tab is divided in three separate

Cardio2D Manual 104 Important: Please pay attention to the "Analyze" dialog. To use the heartbeat average tool, you have to enable the che

Cardio 2D+ Analyzer Software 105 If you like to zoom the Natrium peak of the heartbeats for QT-like analysis, please click the icon "Na-Peak&quo

7 3 Software and Hardware Installation 3.1 Installing the Cardio2D Software System Requirements Software: One of the following Microsoft Windows ®

Cardio2D Manual 106 6.8 Analysis of Field Potential Duration The "Analysis of Field Potential Duration" is also called "QT-related In

Cardio 2D+ Analyzer Software 107 Enable the check box "On" to use the QT-related Analysis tool. Select the "Detection Deadtime" i

Cardio2D Manual 108 Dose Response Curve The right side shows a diagram of the dose response curve, if the recorded data allow to calculate it. The c

109 7 Troubleshooting 7.1 Troubleshooting Most problems occur seldom and only under specific circumstances. In most cases, it is only a minor probl

Cardio2D Manual 110 Your license key is not valid. Please contact Multi Channels Systems MCS GmbH. The minimum rise time has to be shorter than the

111 8 Theoretical Background 8.1 Cardiac Action Potential Over the last 30 years, non-invasive extracellular recording has developed into a widely

Cardio2D Manual 112 Electrocardiogram (Reference: "Estimation of Action Potential Changes from Field Potential Recordings in Multicellular Mous

113 9 Appendix 9.1 Technical Support Please read the "Troubleshooting" part of the user manual or Help first. Most problems are caused by

Cardio2D Manual 114 9.3 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. User forum The Multi

115 10 Index

Cardio2D Manual 8 Installing the Cardio2D Software Make sure to have a valid license for the Cardio2D software! Please ask license@multichannelsystems

Cardio2D Manual 116

117 A Automated Waveform Detection 73 B Basic 31 Blanking Circuit BC 36 Blanking Pulse ... 36 Extension ...

Cardio2D Manual 118 General ... 56 Study ...

119

Software and Hardware Installation 9 3.2 First Use of Cardio2D after Installation Double-click the Cardio2D program icon. The "Data Acquisition

Cardio2D Manual 10 For saving the settings as default, please enter "File" menu "Save Defaults". 3.3 Data Acquisition Hardware

Software and Hardware Installation 11 Data Acquisition with the MEA2100-Systems The MEA2100 recording system is an all-in one solution consisting of h

Cardio2D Manual 12 The USB-ME128-System and the USB-ME64-System are in principle the same devices as the USB-ME256-System, but with 128 or 64 electro

Software and Hardware Installation 13 Data Acquisition with the USB-MEA256-Systems The USB-MEA256-System is a compact and portable stand-alone solutio

Cardio2D Manual 14 MC_Card 64:  MEA_6Well_60MEA_200_30  MEA_8x8_100_10  MEA_8x8_200_30  MEA_8x8_1000_30  MEA_6x10_500_10  MEA_6x10_500_3

Software and Hardware Installation 15 USB-MEA64:  MEA_6Well_60MEA_200_30  MEA_8x8_100_10  MEA_8x8_200_30  MEA_8x8_1000_30  MEA_6x10_500_10

Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express w

Cardio2D Manual 16 USB-ME128:  MEA_6Well_60MEA_200_30  MEA_8x8_100_10  MEA_8x8_200_30  MEA_8x8_1000_30  MEA_6x10_500_10  MEA_6x10_500_30

17 4 Cardio 2D Software 4.1 The Cardio2D Program : Overview Main Window: Overview The main window of Cardio2D consists of two in case of several

Cardio2D Manual 18 Control Window The experimental protocol is visualized by three icons on the left orange control view. The icons can be clicked fo

Cardio 2D Software 19 Main Menu File Menu to open or save templates, to restore and save defaults, and to exit the program. If you design experiment

Cardio2D Manual 20 Tools Menu to activate the offset correction, if necessary. Enable the option "Offset Correction" and the following dial

Cardio 2D Software 21 Click "Data Acquisition" in the Settings menu. The "Data Acquisition Settings" dialog opens. This dialog is

Cardio2D Manual 22 Design of the MEA window when connecting three MEAs, and using a MEA with a 6 x 10 layout grid in MEA_3 tab. Design of the MEA win

Cardio 2D Software 23 Click "View Summary Page" to open the "Summary" window. This window is only available, if more than one MEA

Cardio2D Manual 24 In this example the "Summary" page shows 60-6wellMEAs with adapted color map. Help Menu for starting the Cardio2D onlin

Cardio 2D Software 25 4.2 Control Window The "Control Window" is divided into two window panes. The "Control View" controls the

iii Table of Contents Introduction 1 About this Manual 1 Welcome to Cardio2D and Cardio2D+ 1 Important Information and Instructions 3 Important S

Cardio2D Manual 26 4.3 Control View In the Control window you can enter the settings for setting up and running an experiment. If more than one MEA

Cardio 2D Software 27 4.4 Viability Click Viability. The "Viability" icon changes into "Stop" icon , and the duration of the

Cardio2D Manual 28 For example: Type the desired settings into the "Study" tabbed page of the global labbook selected by the icon in the &qu

Cardio 2D Software 29 4.6 Compound / Basic Compound Click "Compound" in main menu "Experiment". The "Basic" icon is re

Cardio2D Manual 30 In the control window beneath the "Compound" icon, the currently applied dose of compound is displayed. When clicking on

Cardio 2D Software 31 There are different ways to influence the recordings. 1. Select "Timer" in the "Switch" drop down menu and

Cardio2D Manual 32 Apply Phase: The recording time is user defined. The data of the recording phase is saved, the data of the apply phase is excluded

Cardio 2D Software 33 4.7 Stimulation of MEA Electrodes Click "Stimulate" icon . The stimulation feature gives the possibility for voltag

Cardio2D Manual 34 Stimulus Pulse In the "Stimulus" menu you design the stimulus pulse. The pulse shape of a stimulus pulse is usually bip

Cardio 2D Software 35 When using the blanking feature, please enable the "Sync Out" check box. You have to define the time of "Extensio

Cardio2D Manual iv Cardio 2D+ Analyzer Software 69 The Cardio2D+ Analyzer Program: Overview 69 Control Window Cardio2D+ 82 MEA Window Cardio2D+

Cardio2D Manual 36 MEA_Select MEA_Select is a software program for controlling the blanking circuit of the MEA amplifier. With MEA_Select it is possib

Cardio 2D Software 37 4.8 Stimulation with MEA2100-System Click "Stimulate" icon . When working with Cardio2D and any type of MEA2100-Syst

Cardio2D Manual 38 Second section: Click one of the three color coded "Select Stimulus" icons to select the respective stimulus pattern fo

Cardio 2D Software 39 Stimulation Pattern To open the "Define Stimulus 1" dialog, please select the first "Select Stimulus 1" icon

Cardio2D Manual 40 Building Stimulation Patterns On the left side of the upper section you can choose the provided default stimulus pattern. Move the

Cardio 2D Software 41 Modulation of the provided Stimulation Patterns Initially the provided stimulation patterns are default. To adjust a primitive t

Cardio2D Manual 42 Stimulus Pattern: Sine Wave Modulate the amplitude "PP Amp (mV)", the period "Period (μs), the shift "Shif

Cardio 2D Software 43 Stimulus Pattern: Rectangle Modulate the amplitude "Amplitude (mV)" of the ascending and the descend part of th

Cardio2D Manual 44 Stimulus Pattern: Ramp Modulate the amplitude "Amplitude (mV)" and the duration "Duration (μs)” of the ramp pul

Cardio 2D Software 45 Stimulus Pattern: Biological Pulse If you like to use, for example, a biological signal as stimulus pulse, you can import s

1 1 Introduction 1.1 About this Manual This manual comprises all important information about the first installation of the hardware and software,

Cardio2D Manual 46 When using more than one cycle you can click onto the arrow symbol for one additional option: A highlighted parameter field appea

Cardio 2D Software 47 Analyzer Settings Click "Analyze" icon . The "Analyzer Settings" dialog will open. Click "HB Detection

Cardio2D Manual 48 The drop down menu "Min/Max Type" describes the type of the minimum or maximum "Local" or "Global". T

Cardio 2D Software 49 Click "2D" tab in Analyzer Settings. In this dialog you can define the time span from the drop down menu in a rang

Cardio2D Manual 50 4.9 Start / Stop Start Click Start to start recording. The "Start" icon changes into "Stop" icon , and t

Cardio 2D Software 51 4.11 Summary Window Click "View Summary Page" in main menu "Settings" to open or to remove the "Summ

Cardio2D Manual 52 When clicking on the "Summary" button the window will be visible again. 4.12 MEA Window The "MEA Window" is

Cardio 2D Software 53 The "MEA Window" changes depending on the connected hardware. If you connect, for example, an USB-MEA256-System for da

Cardio2D Manual 54 4.14 Single Channel View Click field potential "FP" tab in single channel view. The single channel view allows a closer

Cardio 2D Software 55 Click heartbeat "HB" tab in single channel view. The HB tab shows a diagram of the duration of the RR interval in ms i

Cardio2D Manual 56 Click average "Ave" tab in single channel view. The recordings of one user defined electrode channel are displayed in a

Cardio 2D Software 57 The diagram of Latency window shows the latency (μs) of the excitation calculated between the first and the last detected elect

Cardio2D Manual 58 4.16 Color Map The color map displays the propagation of each heartbeat across the MEA. The isochrones are indicated by black lin

Cardio 2D Software 59 Click the "Propagation Path" icon to set the propagation path. The electrodes on the MEA are symbolized by circles:

Cardio2D Manual 60 Color Map: The usual used color map for cardiac issues is the "Jet Invers" map, but you can also choose "Hot, Cool o

Cardio 2D Software 61 Select "Data" tab in "Map Settings" dialog. Interpolation: In the "Map" tab of the "Map Sett

Cardio2D Manual 62 4.17 General Software Features Tracking Axes For tracking the axis manually, click on the axis and the mouse pointer will change

Cardio 2D Software 63 Click the "Plot" icon . The following toolbar appears. Tracking Scroll axes Zoom axes Zoom in and zoom out

Cardio2D Manual 64 Click "Edit" icon . The following dialog appears. Now it is possible to format the plot.

65 5 Experimental Proceeding 5.1 General Performance / Noise Level Please use the provided model test probe to test the amplifier immediately after

3 2 Important Information and Instructions 2.1 Important Safety Advice Warning: Make sure to read the following advice prior to install or to use

Cardio2D Manual 66 5.2 Detection Parameter Under the tab "HB Detection" in "Analyzer Settings" of the MEA electrodes plate of t

Experimental Proceeding 67 The sodium current is detected by the rapid change in the field potential, indicated by the rise time. The rise time refers

Cardio2D Manual 68 Slope Calculation The calculation of the slope of each data point (P) is achieved by linear regression. This linear regression is p

69 6 Cardio 2D+ Analyzer Software 6.1 The Cardio2D+ Analyzer Program: Overview Main Window: Overview The main window of Cardio2D+ is divided in t

Cardio2D Manual 70 MEA Window The window on the right side is divided into four separate window panes and has five tabs: "Field Potential",

Cardio 2D+ Analyzer Software 71 Select "ROI Selection" tab. Please see the picture below. This tabbed page lists the single recording seque

Cardio2D Manual 72 The color map corresponds with the single channel view, and shows the excitation patterns of that heartbeat marked with the blue a

Cardio 2D+ Analyzer Software 73 The "MEA electrode field" window corresponds directly with the "Channel overlay" view. The field

Cardio2D Manual 74 Select one channel from the "Heartbeat" window pane of the "Control Window" and the desired channel will be dis

Cardio 2D+ Analyzer Software 75 Main Menu File Menu to open or save result files, to open raw data files, and to exit the program. Cardio2D generate

Cardio2D Manual 4 2.2 Guarantee and Liability The General conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply. The operato

Cardio2D Manual 76 Click "HB Detection" tab in the "Analyze" dialog. In this dialog you define the parameter for detecting the wa

Cardio 2D+ Analyzer Software 77 The drop down menu "Min/Max Type" describes the type of the minimum or maximum "Local" or "Gl

Cardio2D Manual 78 Click "HB Average" tab in "Analyze" dialog. If the heartbeat average feature is welcome, please enable the che

Cardio 2D+ Analyzer Software 79 Click "2D" tab in "Analyze" dialog. The first up down box in this tab of the dialog defines the m

Cardio2D Manual 80 Click "QT" tab in "Analyze" dialog. This dialog defines the parameters for the QT-related Analysis. QT-related

Cardio 2D+ Analyzer Software 81 Help Menu for activating the online help for Cardio2D+, and for displaying the dialog "About Cardio2D+".

Cardio2D Manual 82 6.2 Control Window Cardio2D+ The control window of Cardio2D+ is divided in three separate windows: The "Recording control&quo

Cardio 2D+ Analyzer Software 83 Recording Control of Cardio2D+ In the recording control window the name(s) of the currently analyzed Cardio2D raw dat

Cardio2D Manual 84 In this screenshot a MEA electrode grid 8 x 8 is displayed. Different layout grids are possible, depending on the selected raw data

Cardio 2D+ Analyzer Software 85 6.4 Field Potential Cardio2D+ Click "Field Potential" tab. The "Field Potential" tab is divided

Important Information and Instructions 5 2.4 Terms of Use for the Program You are free to use the program for its intended purpose. You agree that yo

Cardio2D Manual 86 Click "Edit Plot" icon . A plot menu appears. With the help of the plot menu it is possible to modify a lot of plot par

Cardio 2D+ Analyzer Software 87 MEA Electrode Field Recorded data traces are displayed in the MEA electrode field. To adapt the y-axis, please use the

Cardio2D Manual 88 Channel Overlay If you select one or more than one MEA electrodes in the MEA electrode field, they are displayed in an overlay plot

Cardio 2D+ Analyzer Software 89 Color Map The color map displays the field potentials on the MEA electrodes at a time stamp, defined by the channel ov

Cardio2D Manual 90 Click the "Map Settings" icon . The dialog "Map Settings" appears. Click "Map" tab. In this dialog

Cardio 2D+ Analyzer Software 91 Auto: Click the check box "Auto" in "Maximum" option. The "Minimum" is zero, you are not

Cardio2D Manual 92 Click the "Record Movie" icon . The dialog "Movie Recorder" appears. The movie of the color map will be reco

Cardio 2D+ Analyzer Software 93 6.5 ROI Selection Click "ROI Selection" tab.

Cardio2D Manual 94 The "ROI Selection" tabbed page lists the single recording sequences from one electrode. Select the electrode in the &quo

Cardio 2D+ Analyzer Software 95 6.6 Heartbeat Cardio2D+ Click "Heartbeat" tab. The "Field Potential" tab is divided in four sepa